[PDF] RNase Y in Bacillus subtilis: a Natively Disordered Protein That Is the Functional Equivalent of RNase E from Escherichia coli | Semantic Scholar (2024)

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@article{LehnikHabrink2011RNaseYI, title={RNase Y in Bacillus subtilis: a Natively Disordered Protein That Is the Functional Equivalent of RNase E from Escherichia coli}, author={Martin Lehnik‐Habrink and Joseph A. Newman and Fabian M Rothe and Alexandra S. Solovyova and Cecilia Rodrigues and Christina Herzberg and Fabian M. Commichau and Richard James Lewis and J{\"o}rg St{\"u}lke}, journal={Journal of Bacteriology}, year={2011}, volume={193}, pages={5431 - 5441}, url={https://api.semanticscholar.org/CorpusID:12507075}}
  • Martin Lehnik‐Habrink, J. Newman, J. Stülke
  • Published in Journal of Bacteriology 29 July 2011
  • Biology

Novel evidence is provided for the idea that RNase Y is the functional equivalent of RNase E, even though the two enzymes do not share any sequence similarity.

103 Citations

Highly Influential Citations


Background Citations


Methods Citations


Results Citations


103 Citations

Localization of Components of the RNA-Degrading Machine in Bacillus subtilis
    N. Cascante-EstepaK. GunkaJ. Stülke


    Front. Microbiol.

  • 2016

The findings suggest that the interactions of the enzymes involved in RNA degradation in B. subtilis are rather transient, and the bulk of these enzymes is located in the cytoplasm.

  • 38
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RNA degradation in Bacillus subtilis: an interplay of essential endo‐ and exoribonucleases
    Martin Lehnik‐HabrinkR. LewisU. MäderJ. Stülke


    Molecular microbiology

  • 2012

A model, based on recent published data, of RNA degradation in B. subtilis mRNAs is presented, which shows that degradation is initiated by RNase Y‐dependent endonucleolytic cleavage, followed by processive exoribon nucleolysis of the generated fragments both in 3‐to‐5′ and in 5‐to-3′ directions.

Localization and Function of RNases in Bacillus subtilis
    N. Cascante-Estepa


  • 2017

The effect of citrate, c-di-GMP and c- di-AMP, as well as enolase and phosphofructokinase, on the RNA degradation activity of PNPase is studied, which confirms the interaction of the RNases J1 and J2 through the C-terminal domain, and shows that they oligomerize as dimers and tetramers.

  • Highly Influenced
Molecular and genetic interactions of the RNA degradation machineries in Firmicute bacteria
    Peter Redder


    Wiley interdisciplinary reviews. RNA

  • 2018

An overview of the data on interactions between Firmicute RNA degradation factors is gathered, to highlight the similarities and differences between experimental data from different experiments and from different organisms.

  • 17
  • Highly Influenced
Bacillus subtilis RNase Y Activity In Vivo Analysed by Tiling Microarrays
    S. LaalamiP. BessièresA. RoccaL. ZigP. NicolasH. Putzer


    PloS one

  • 2013

The data confirm that this endoribonuclease plays a key role in initiating the decay of a large number of mRNAs as well as non coding RNAs and confirm already known RNase Y substrates.

Maturation of polycistronic mRNAs by the endoribonuclease RNase Y and its associated Y-complex in Bacillus subtilis
    Aaron DeLougheryJean-Benoît LalanneR. LosickGene-Wei Li


    Proceedings of the National Academy of Sciences

  • 2018

It is found that the Y-complex is required for the majority of RNase Y-mediated mRNA maturation events and also affects riboswitch abundance in B. subtilis, and proposed that its role is conserved in Staphylococcus aureus.

  • 55
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Dissection of the network of interactions that links RNA processing with glycolysis in the Bacillus subtilis degradosome.
    J. NewmanL. HewittC. RodriguesA. SolovyovaC. HarwoodR. Lewis


    Journal of molecular biology

  • 2012
  • 57
Examining the Protein Interactome and Subcellular Localization of RNase J2 Complexes in Streptococcus mutans
    Rong MuPushkar ShindeZhengzhong ZouJ. KrethJ. Merritt


    Front. Microbiol.

  • 2019

The results suggest that S. mutans degradosomes are either unlikely to exist or are quite distinct from those of E. coli, andRNase J2 exhibits multiple novel interactions that have not been previously reported for any RNase J proteins, some of which were highly biased for either the cytoplasmic or membrane fractions.

  • 6
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In Vitro Study of the Major Bacillus subtilis Ribonucleases Y and J.
    L. MoraS. NgoS. LaalamiH. Putzer

    Biology, Chemistry

    Methods in enzymology

  • 2018
  • 4
Phase‐separated bacterial ribonucleoprotein bodies organize mRNA decay

This review will highlight the functional role that BR‐bodies play in the mRNA decay process through its organization into a membraneless organelle in the bacterial cytoplasm, and suggest that these phase‐separated structures are broadly distributed across bacteria, and in evolutionarily related mitochondria and chloroplasts.

  • 15
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63 References

The RNA degradosome in Bacillus subtilis: identification of CshA as the major RNA helicase in the multiprotein complex
    Martin Lehnik‐HabrinkH. PförtnerLeonie RempetersNico PietackC. HerzbergJ. Stülke


    Molecular microbiology

  • 2010

The results suggest that CshA is the functional equivalent of the RhlB helicase of the Escherichia coli RNA degradosome.

  • 135
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The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E.
    A. J. Carpousis


    Annual review of microbiology

  • 2007

RNA degradosome-like complexes appear to be conserved throughout the Proteobacteria, but there is a surprising variability in composition that might contribute to the adaptation of these bacteria to the enormously wide variety of niches in which they live.

  • 446
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RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y
    Martin Lehnik‐HabrinkM. SchafferU. MäderChristine DiethmaierC. HerzbergJ. Stülke


    Molecular microbiology

  • 2011

The results presented in this work emphasize the importance of RNase Y as the global acting endoribonuclease for B.‚subtilis as well as other Gram‐positive bacteria.

  • 97
RNA Processing and Degradation in Bacillus subtilis
    C. Condon


    Microbiology and Molecular Biology Reviews

  • 2003

This review focuses on the enzymes and pathways of RNA processing and degradation in Bacillus subtilis, and compares them to those of its gram-negative counterpart, Escherichia coli, to reveal that B. subtili has a very different selection of RNases available for RNA maturation.

  • 158
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RNA degradosomes exist in vivo in Escherichia coli as multicomponent complexes associated with the cytoplasmic membrane via the N-terminal region of ribonuclease E.
    G. LiouW. JaneStanley N. CohenNa-Sheng LinS. Lin-Chao


    Proceedings of the National Academy of Sciences…

  • 2001

RNase E isolated from Escherichia coli is contained in a multicomponent "degradosome" complex with other proteins implicated in RNA decay. Earlier work has shown that the C-terminal region of RNase E

  • 160
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RNase Y, a novel endoribonuclease, initiates riboswitch turnover in Bacillus subtilis
    K. ShahbabianAilar JamalliL. ZigH. Putzer


    The EMBO journal

  • 2009

RNase Y might be not only important for riboswitch RNA turnover but also as a key player in the initiation of mRNA decay in B. subtilis.

  • 228
  • Highly Influential
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The endoribonucleolytic N-terminal half of Escherichia coli RNase E is evolutionarily conserved in Synechocystis sp. and other bacteria but not the C-terminal half, which is sufficient for degradosome assembly.
    V. KaberdinA. MiczakJ. S. JakobsenS. Lin-ChaoJ. McDowallA. Gabain


    Proceedings of the National Academy of Sciences…

  • 1998

It is shown here that the sequence of the N-terminal endoribonucleolytic domain of RNase E is evolutionarily conserved in Synechocystis sp.

  • 138
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Endonucleolytic initiation of mRNA decay in Escherichia coli.
    A. J. CarpousisB. LuisiK. McDowall


    Progress in molecular biology and translational…

  • 2009
  • 149
Global analysis of Escherichia coli RNA degradosome function using DNA microarrays.
    J. BernsteinPei-Hsun LinStanley N CohenS. Lin-Chao


    Proceedings of the National Academy of Sciences…

  • 2004

The results, which argue that decay of some E. coli mRNAs in vivo depends on the action of assembled degradosomes, whereas others are acted on by de gradosome proteins functioning independently of the complex, imply the existence of structural features or biochemical factors that target specific classes of m RNAs for decay by de Gradosomes.

  • 235
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The RNase E of Escherichia coli is a membrane‐binding protein
    Vanessa KhemiciL. PoljakB. LuisiA. J. Carpousis

    Biology, Chemistry

    Molecular microbiology

  • 2008

This is the first report demonstrating that RNase E is a membrane‐binding protein and that its localization to the inner cytoplasmic membrane is important for normal cell growth.

  • 198
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    [PDF] RNase Y in Bacillus subtilis: a Natively Disordered Protein That Is the Functional Equivalent of RNase E from Escherichia coli | Semantic Scholar (2024)


    What is the difference between E coli and Bacillus subtilis? ›

    The differences between the E. coli and B. subtilis enzymes are determined by the core component of the enzyme in vitro, but the exact structural determinants responsible for the altered signal recognition are difficult to pinpoint due to a substantial divergence of these two proteins.

    What is the function of ribonuclease Y? ›

    RNase Y of Staphylococcus aureus plays a major role in virulence gene regulation and is involved in the processing and stabilization of a global regulator system, SaePQRS (14). These observations suggest that RNase Y is the major endoribonuclease in mRNA degradation in B.

    Does Bacillus subtilis have RNA? ›

    B. subtilis RNase P is a heterotetramer consisting of two protein subunits (encoded by rnpA) and two RNA subunits (encoded by rnpB) (65). The RNA subunit contains the catalytic site and, as such, constitutes a ribozyme (82), while the protein subunit facilitates substrate recognition (48, 120, 196).

    Where are the endospores located in Bacillus subtilis? ›

    Endospores may be located in a terminal (end of the cell), subterminal (near the end of the cell), or central (middle of the cell) position.

    Is Bacillus subtilis good or bad for you? ›

    "Good" bacteria such as B. subtilis might help the body break down food, absorb nutrients, and fight off "bad" organisms that might cause diseases. These bacteria are sometimes added to fermented foods like yogurt and also found in dietary supplements.

    What is special about Bacillus subtilis? ›

    Bacillus subtilis exhibits both a direct and indirect biocontrol mechanism to suppress disease caused by pathogens. The direct mechanism includes the synthesis of many secondary metabolites, hormones, cell-wall-degrading enzymes, and antioxidants that assist the plant in its defense against pathogen attack.

    What does RNase do to bacteria? ›

    RNase E is an essential endoribonuclease involved in most aspects of RNA processing and degradation in many bacteria. It is active as a tetramer within the context of a membrane-bound RNA degradosome complex, which contains other enzymes involved in RNA and cellular metabolism in addition to RNase E.

    What is the most common RNase? ›

    RNase 7 was first detected and purified from human skin; in fact, it is the most abundant RNase found in the skin and is constitutively expressed.

    What is the function of protein Y? ›

    One possible answer is that protein Y binds protein X and sequesters it by keeping protein X in some subcellular compartment, away from the nucleus.

    Is Bacillus subtilis a virus or bacteria? ›

    Bacillus subtilis is a ubiquitous bacterium commonly recovered from water, soil, air, and decomposing plant residue. The bacterium produces an endospore that allows it to endure extreme conditions of heat and desiccation in the environment.

    Is Bacillus subtilis a bacteria or fungi? ›

    B. subtilis is a rod-shaped bacterium, which produces endospores that allow the survival of extreme environmental conditions including heat and desiccation. In the soil, the natural environment of B.

    What kind of bacteria is Bacillus subtilis? ›

    B. subtilis is a fast-growing, Gram-positive, aerobic bacterium with rod-shaped cells that are typically 2–6 µm long and just less than 1 µm in diameter. The optimal growth temperature is about 30–35 C, giving a doubling time of as little as 20 min.

    Where is Bacillus subtilis found in the human body? ›

    subtilis is found in soil and the gastrointestinal tract of ruminants and humans. Interesting Facts: B. subtilis is considered the best studied Gram-positive bacterium and a model organism in the study of bacterial chromosome replication and cell differentiation.

    Where is Bacillus subtilis obtained from? ›

    Bacillus subtilis, a low %G+C, Gram-positive, endospore-forming member of the bacterial phylum Firmicutes, is found predominately in the soil and in association with plants.

    Is Bacillus subtilis aerobic or anaerobic? ›

    The Gram-positive soil bacterium Bacillus subtilis, generally regarded as an aerobe, grows under strict anaerobic conditions using nitrate as an electron acceptor and should be designated as a facultative anaerobe.

    What is the difference between E. coli and Bacillus? ›

    coli system. Bacillus subtilis as a probiotic has various advantages compared to E. coli as it is safe and nontoxic, can secrete foreign proteins, can survive in harsh environmental conditions to which E. coli cannot adapt, and can improve animal gut health (Table 1). ...

    What is another name for Bacillus subtilis? ›

    Bacillus subtilis(/bəˈsɪl. əs subˈtiː. lis/), known also as the hay bacillus or grass bacillus, is a gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants, humans and marine sponges. As a member of the genus Bacillus, B.

    Is E. coli a Bacillus bacteria? ›

    Escherichia coli (E. coli) is a gram-negative bacillus known to be a part of normal intestinal flora but can also be the cause of intestinal and extraintestinal illness in humans. There are hundreds of identified E.

    Is E. coli a bacillus? ›

    Escherichia coli (E. coli) is a gram-negative bacillus that is a causative organism of many diarrheal illnesses, including traveler's diarrhea and dysentery.


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